THE EFFECT OF URINE STORAGE TIME ON ROOM TEMPERATURE ON THE NUMBER AND MORPHOLOGY OF URINE LEUCOCYTES WITH MICROSCOPIC URINALYSIS BY OBSERVING URINE SEDIMENT

Abstract

Urinalysis is also known as a screening test. Urinalysis is recommended no later than 2 hours from the time the urine is voided. Delaying urinalysis for 2 hours can reduce the quality of the results of examination of urine organic elements including erythrocytes, leukocytes and bacteria. The purpose of this study was to determine the effect of storage time of urine at room temperature on the number and morphology of urine leukocytes using an examination time span of 1 hour, 2 hours and 3 hours. The type of research used is experimental. Research method used microscopic urinalysis by observing urine sediment. The sample used is morning urine as many as 30 samples. The results showed that the average number of leukocytes during the 1 hour delay was 27.60/LPB with the morphology of the leukocytes being round, having a multilobed nucleus and being granular; a delay of 2 hours of 19.60/LPB with a leukocyte morphology that is not perfectly round and its size is reduced; and for 3 hours of 9.47/LPB with morphological changes in the size of the leukocytes that are getting smaller and their shape is almost amorphous. Results of the test One-way ANOVA, obtained p value of 0.000, which means that there is an effect of storage time of urine at room temperature on the number and morphology of urine leukocytes