Comparisson Of The Examination Of Mycobacterium Tuberculosis (M.Tb) Using Polymerase Chain Reaction (PCR) And ZiehlNeelsen (ZN) On Tuberculosis (Tb) Patients Suspect In Public Health Facilities Of Manado

Abstract

(TB) is an infectious disease caused by Mycobacterium tuberculosis. TB infections occur
through the air, namely the inhalation of droplets containing germs from an infected person. Four
People exposed with Mycobacterium tuberculosis have a 10% risk of developing pulmonary TB.
The aim of this research was to compare of the examination of M.Tb using polymerase chain
reaction and ziehlneelsen on TB Patients suspect in Public Health Center of Manado. The
research method used was analytic laboratory examination. As the result, TB examination using
ZiehlNeelsen method found 13 samples with positive TB, while using the PCR examination
method 93 samples (93,6%) showed positive. There was significantly differences between PCR
and ZiehlNeelsen examination method. TB examination using the PCR method had high
sensitivity and specificity to detect Mycobacterium tuberculosis, so that more MTB bacteria can
be detected than using the ZN test. It is recommended that health workers at the Public Health
center to diagnose TB should use an examination that has high sensitivity and specificity so that
the results obtained are more accurate.