ANTIBIOFILM DETERMINATION OF Curcuma longa L. RHIZOME AS DRUG CANDIDATES IN HANDLING Propionibacterium acnes RESISTANCE

Abstract

Propionibacterium acnes is the cause of acne vulgaris which is characterized by comedones, papules, pustules, and nodules. The problem of antibiotic resistance against P.acnes is increasing, so we need compounds that can inhibit the formation of biofilms, one of which is Curcuma longa L. This study aims to determine the antibiofilm activity from the active fraction of Curcuma longa L. against P. acnes. The analytical method used is descriptive quantitative. The analysis method included test of extract parameters, determination of curcuminoid levels, antibacterial and antibiofilm activity from the active fraction of Curcuma longa L., and GC-MS analysis. The results showed that the extract parameters test consisted of organoleptic, drying shrinkage (9.2%), and water content (5%), appropriate to Indonesian herbal pharmacopoeia standards. Antibacterial activity test of both extract and fraction Curcuma longa L., obtained the highest value in the methanol fraction. The activity results test of the methanol fraction obtained bacteriostatic activity in the concentration range of 0.5-0.75%, while at a concentration of 1-5% having bactericidal activity. The P.acnes antibiofilm test had an inhibition value of 0.25% with a minimum inhibitory concentration of biofilm (MICB) of 0.5%. The main compound identified in the active methanol fraction was aR-turmerone. In conclusion, the rhizome of Curcuma longa L. has potential as an antibiofilm against P. acnes, especially the aR-turmerone compound which is the main component of the essential oil of Curcuma longa L.