ACCURACY AND PRECISION OF URIC ACID EXAMINATION POINT OF CARE TESTING METHOD AND URICASE ENZYMATIC COLORIMETRIC METHOD

Authors

  • Fitria Yulfirda Arini Poltekkes of the Ministry of Health Surabaya
  • Anik Handayati Poltekkes of the Ministry of Health Surabaya
  • Ayu Puspitasari Poltekkes of the Ministry of Health Surabaya

Abstract

The method that often used in clinical chemistry examinations in laboratory such as analysis of blood glucose, uric
acid, cholesterol, triglycerides, bilirubin, and albumin are POCT method and the uricase enzymatic colorimetric
method. The POCT method is a automatic method that quickly obtains result. While the uricase enzymatic
colorimetric method is a manual method that still requires special treatments to obtain result. In this study, the
parameters of uric acid examination were chosen because uric acid is a rheumatic disease that ranks third after
arthrosis and rheumatoid arthritis. It was estimated that almost 80% of the population aged 40 years or older have
gout sufferers in Indonesia. Therefore, a study was conducted on the accuracy and precision of the POCT method
and the uricase enzymatic colorimetric method, which aims to determine the accuracy and precision of uric acid
examination POCT method and uricase enzymatic colorimetric method. The data analysis used is descriptive
analysis to analyze the value of accuracy and precision. As the results of this research, 12.30% of bias value and
110.88% of recovery value were generated. While the coefficient of variation from the POCT method were 3.5%
and the uricase enzymatic colorimetric method were 5.37%. Based on these results, it could be concluded that the
accuracy and precision of uric acid examination using the POCT method and the uricase enzymatic colorimetric
method were equally good and both method provided trusted results.

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Published

2021-10-05